Intern special Learn " Sampling " ----------------------- Venous Blood Sampling **

Intern special
Learn " Sampling "
-----------------------
Venous Blood Sampling
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1- Apply a tourniquet proximal to the
venepuncture site.
2- Tap on or rub the skin overlying the
vein you want to puncture lightly to
make it standout; or if the vein is
obscure, you may be able to palpate its
course with you index finger.
3- Sterilization with alcohol and leave to
dry in air
(traces of alcohol causes hemolysis)
4- Hold the syringe between the thumb
and the middle, ring and small finger of
your dominant hand and steady the
needle using your index finger
.
" Never hold the syringe like a pencil because it limits your angle of skin
entry "
5- confirme entry of the vein by
aspiration of blood, Once the desired
volume of blood is aspirated into the
syringe, steady it with one hand and
release the tourniquet with the other
hand.
6- Use a piece of dry gauze to apply
gentle pressure to the entry site while
the needle is withdrawn. Ask the
patient to continue to apply pressure
while you attend to the specimen
obtained .
7- Remove the needle from the syringe
and drop it into the special box for
disposal of sharp instruments
immediately .
8- Uncap the specimen container and
deposit the blood sample into it directly
from the syringe. Injecting blood into
the container via the needle is not
advisable because:
(a) you may prick
yourself and
(b) forcing blood through the needle may cause hemolysis that
can adversely affect the analytical
fitness of the specimen.
■ Avoid hemolysis:-
Allow alcohol to dry
Clean tubes
Withdraw blood slowly
Do not use too fine needle
Deliver blood gently to the tube Avoid frothing
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